A whole slide image (WSI) is accomplished by digital scanning and allows online viewing of your entire stained section.
The VS200 can scan histology slides stained for brightfield imaging (H&E, PAS, special stains) or with fluorescent antibodies. Standard 1″x3″ slides and larger 2″x3″ slides can be scanned. Can scan up to 210 standard slides automatically.
More information about the MPC Olympus VS200 can be found here. This scanner was awarded through an NIH Shared Instrumentation Grant and installed in November 2023.
VS200 scanner components:
Objectives:
PLAN APO 2X OBJECTIVE, NA 0.08, WD 6.2MM
UPLXAPO 4X OBJECTIVE, OFN26.5, NA0.16, WD13mm
UPLXAPO 20X OBJECTIVE, OFN26.5, NA0.80, WD0.6mm
UPLXAPO 40X OBJECTIVE, OFN26.5, NA0.95, WD0.18mm
Lightsource: X-Cite NOVEM-L (XT920)
Filter cubes (7 fluorescent color channels and brightfield):
DAPI/Hoechst/Alexa350 Filter Set 375/28X, BS415
EGFP Filter Set 470/40X, BS495, 525/50M
CY3/TRITC Filter Set 545/25X, BS565, 605/70M
mCherry/TxRed Filter Set 560/40X, BS585, 630/75M
CY5 Filter Set 620/60X, BS660, 700/75M
CY7 Filter Set 710/75X, BS760, 810/90M
Nuclear Yellow Filter Set 350/50X, BS470, 500/40M
Multiband filter set, 25mm, optimized for DAPI, FITC, TRITC, Cy5, & Cy7
Image analysis programs will be available on a dedicated analysis workstation running VS200 desktop, 3D deconvolution, deep neural network, and VSI image conversion software.
Submit Slides
- Submit the online Whole Slide Scanning form.
- Drop off slides at the front D11-41 submissions counter.
- Turnaround time is 0.5-2 work days depending on the total slide number and current workload.
- Let staff know of any deadlines at the time of slide submission.
- Install viewing software (see next section)
- You will receive an email when the WSI files are ready for transfer using DropBox. Slides can be picked up at the D11-41 submissions counter.
View Scans- Freeware
Each slide scan will have a folder and thumbnail views of slide label and scanned area.
- Please acknowledge funding as follows: The Olympus VS200 was purchased with a shared instrumentation grant from the Office of the Director at the National Institutes of Health (S10OD032236).
Prepare Slides for scanning
Best scanning results will be obtained from glass slides with #1.5 thickness coverslips and regular thickness (4-5 microns) tissues. Slides with marks (such as ink dots), extra mounting medium, non-glass slides, plastic coverslips, thick labels, and labels that are going around the slide present challenges for automatically finding the tissue and proper imaging. These artifacts may need to be removed to obtain an optimal scan. Images are individually reviewed once the scanning is completed. Scans with poor quality will be rescanned manually to achieve the maximum quality possible.
Slides must be completely dry before scanning. The equipment can not scan slides with wet mounting media, more than two slide labels, labels on the back or the bottom of the slide, double slides, or broken slides. The slide and coverslip should be free of fingerprints, dust, and dirt. Any pen marker on the slide also need to be removed as the scanner will consider such marks as tissue. Faint stains, air bubbles, thick sections (> 50um), or marks on the glass surface can cause lower scan quality. In some cases, sample recut or restaining may be recommended.
Scanning Fees and Invoicing
Please visit the Fee page for information on costs and billing.
- Olympus software for VS200 OlyVIA
- FIJI/ImageJ
- QuPATH
Publications
- Please acknowledge funding as follows: The Olympus VS200 was purchased with a shared instrumentation grant from the Office of the Director at the National Institutes of Health (S10OD032236).
Prepare Slides for scanning
Best scanning results will be obtained from glass slides with #1.5 thickness coverslips and regular thickness (4-5 microns) tissues. Slides with marks (such as ink dots), extra mounting medium, non-glass slides, plastic coverslips, thick labels, and labels that are going around the slide present challenges for automatically finding the tissue and proper imaging. These artifacts may need to be removed to obtain an optimal scan. Images are individually reviewed once the scanning is completed. Scans with poor quality will be rescanned manually to achieve the maximum quality possible.
Slides must be completely dry before scanning. The equipment can not scan slides with wet mounting media, more than two slide labels, labels on the back or the bottom of the slide, double slides, or broken slides. The slide and coverslip should be free of fingerprints, dust, and dirt. Any pen marker on the slide also need to be removed as the scanner will consider such marks as tissue. Faint stains, air bubbles, thick sections (> 50um), or marks on the glass surface can cause lower scan quality. In some cases, sample recut or restaining may be recommended.
Scanning Fees and Invoicing
Please visit the Fee page for information on costs and billing.